elisa tests tesee tm sap combi kit  (Bio-Rad)

Journal: PLOS ONE

Article Title: Are rapid tests and confirmatory western blot used for cattle and small ruminants TSEs reliable tools for the diagnosis of Chronic Wasting Disease in Europe?

doi: 10.1371/journal.pone.0286266

Figure Lengend Snippet: Results obtained from the set 1 of samples by the three ELISA rapid tests at NVI. The O.D. represents the mean of duplicates values obtained from each dilution sample. The values above the cut-off indicate positive sample and those below the cut-off indicate negative sample.

Article Snippet: The first set was analysed with all commercially available ELISA tests TeSeE TM SAP Combi Kit (Bio-Rad), TeSeE TM Sheep/Goats (Bio-Rad), HerdChek BSE-Scrapie Ag test (IDEXX) and HerdChek CWD Ag test (IDEXX).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLOS ONE

Article Title: Are rapid tests and confirmatory western blot used for cattle and small ruminants TSEs reliable tools for the diagnosis of Chronic Wasting Disease in Europe?

doi: 10.1371/journal.pone.0286266

Figure Lengend Snippet: Results obtained from the set 2 of samples analyzed at NVI and IRL using three ELISA rapid tests and two western blot methods. The O.D. represents the mean of duplicates /triplicates values obtained from each dilution sample by rapid tests. The values above the cut-off indicate positive sample and those below the cut-off indicate negative sample. Pos and Neg indicate positive and negative results by Western blot analyses.

Article Snippet: The first set was analysed with all commercially available ELISA tests TeSeE TM SAP Combi Kit (Bio-Rad), TeSeE TM Sheep/Goats (Bio-Rad), HerdChek BSE-Scrapie Ag test (IDEXX) and HerdChek CWD Ag test (IDEXX).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Scientific Reports

Article Title: Use of different RT-QuIC substrates for detecting CWD prions in the brain of Norwegian cervids

doi: 10.1038/s41598-019-55078-x

Figure Lengend Snippet: Demographic information and TeSeE TM WB results of the Norwegian animals included in this study.

Article Snippet: Rapid test TeSeE TM SAP ELISA (Bio-Rad) was carried out according to the manufacturer’s instruction.

Techniques:

Journal: Scientific Reports

Article Title: Use of different RT-QuIC substrates for detecting CWD prions in the brain of Norwegian cervids

doi: 10.1038/s41598-019-55078-x

Figure Lengend Snippet: TeSeE TM WB results of brain and lymph nodes collected from the healthy (black) and CWD affected (red) animals included in the study. ( a ) PrP res was detected in all brain homogenates of CWD affected moose (Mo1, Mo2, Mo3), red deer (Rd1) and 3 brain homogenates (out of 7) of CWD affected reindeer. Notably, no PrP res was detected in the brains of CWD affected reindeer 4, 5, 6, and 7; ( b ) PrP res was however detected in the lymph nodes of all the CWD affected reindeer. PrP res was not found in brain and lymph nodes of healthy animals. Numbers on the left of the Western blots indicate molecular weights (kDa).

Article Snippet: Rapid test TeSeE TM SAP ELISA (Bio-Rad) was carried out according to the manufacturer’s instruction.

Techniques: Western Blot